piwik-script

English Intern
Institut für Organische Chemie

MS- und NMR-Analytik

Teamleitung

Dr. Matthias Grüne

Tel.: +49 931 31 85309
matthias.gruene@uni-wuerzburg.de

Lebenslauf und Publikationsliste

Team

Massenspektrometrie

Dr. Juliane Adelmann (Ansprechpartnerin)
Tel. +49 931 31-83618
juliane.adelmann@uni-wuerzburg.de

Antje Heckmann
Tel.  +49 931 31-83184
antje.hautzinger@uni-wuerzburg.de

Michaela Schraut
Tel.  +49 931 31-85977
michaela.schraut@.uni-wuerzburg.de

NMR-Spektrometrie

Patricia Altenberger
Tel.: +49 931 31 88154
patricia.altenberger@uni-wuerzburg.de

Dr. Matthias Grüne (Ansprechpartner)
Tel.: +49 931 31 85309
matthias.gruene@uni-wuerzburg.de

Stefanie Schmitt
Tel.: +49 931 31 83207
stefanie.schmitt1@uni-wuerzburg.de


Equipment

Bruker Avance III HD 600 (‘nmr600a‘)

  • Single bay, installation 2014
  • Oxford magnet, installation 1994
  • SampleXpress automatic sample changer
  • BCU 1 variable temperature precooling unit
  • Cryo platform
  • DCH 13C / 1H cryoprobe with z gradient and ATM

Main applications:

  • 1D and 2D NMR experiments with high sensitivity (cryoprobe), 0 to 80 °C

Bruker Avance III HD 600 (‘nmr600b‘)

  • Double bay, installation 2014
  • Bruker Ascend magnet, installation 2014
  • BACS 120 automatic sample changer
  • BCU 2 variable temperature precooling unit for sample temperatures down to -55 °C
  • Liquid nitrogen heat exchanger for lowest sample temperatures
  • BBFO standard probe with z gradient and ATM, 15N to 31P + 19F / 1H, -150 to +150 °C
  • 10 mm 13C / 1H probe with z gradient and ATM, long term measurements up to 200 °C
  • Equipment for diffusion measurements with pulsed field gradients up to 1800 G/cm: DIFF30L probe (1H inverse / 13C) and GREAT 60 A gradient amplifier
  • 3.2 mm MAS DVT probe, 15N to 31P / 1H + 19F
  • 1.3 mm MAS DVT probe, 15N to 31P + 14N / 1H + 19F

Main applications:         

  • 1D and 2D NMR experiments including heteronuclei
  • Variable temperature measurements
  • DOSY (i.a. large aggregates)
  • Solid state NMR (MAS)

Spare probes for 600 MHz (purchase before 2005):

  • BBO probe with z gradient and ATM, 15N to 31P / 1H
  • TXI probe (1H inverse / 13C / 15N) with z gradient, without ATM
  • Dual probe (19F / 1H) without gradient, without ATM
  • 10 mm BBO probe without gradient, without ATM, 109Ag to 31P / 1H

Bruker Avance III HD 400 (‘nmr400a‘)

  • Nanobay, installation 2014
  • Bruker Ultrashield magnet, installation 2000
  • BACS 60 automatic sample changer
  • BCU 05 variable temperature precooling unit
  • BBFO standard probe with z gradient and ATM, 15N to 31P + 19F / 1H, -150 to +150 °C

Main application:

1D and 2D NMR experiments including heteronuclei
(automation with ICON-NMR software)

Bruker Avance III HD 400 (‘nmr400b‘)

  • Nanobay, installation 2013
  • Bruker Ascend magnet, installation 2013
  • BACS 120 automatic sample changer
  • BCU 2 variable temperature precooling unit for sample temperatures down to -55 °C
  • Liquid nitrogen heat exchanger for lowest sample temperatures
  • BBFO standard probe with z gradient and ATM, 15N to 31P + 19F / 1H, -150 to +150 °C

Main applications:         

  • 1D and 2D NMR experiments including heteronuclei
    (automation with ICON-NMR software)
  • Variable temperature measurements

Installation: 2005

Ionization Techniques

  • Electrospray Ionization (ESI)
  • Atmospheric Pressure Chemical Ionization (APCI)

Specifications

  • Resolution: 18.000 FWHM
  • Mass accuracy: < 3 ppm

Molecules

  • (small) organic compounds
  • Proteins, Peptides
  • Oligonucleotides
  • Oligosaccharides
  • Supramolecular compounds

Applications

  • Direct infusion
  • Fully automated measurements (for self-measurements by OC members after introduction)
  • LCMS (with Agilent 1100 HPLC)
  • DIP-MS (e.g. for direct measurement of solid samples)

Installation: 2017

Ionization Technique

Matrix Assisted Laser Desorption Ionisation (MALDI)

Specifications

  • Resolution: > 40.000 FWHM (reflector TOF)
  • Mass accuracy: < 5 ppm (with external calibration)
  • Resolution: > 1.200 FWHM (linear TOF)
  • Mass accuracy: < 60 ppm (with external calibration)

Molecules

  • Polymers
  • (small) organic compounds
  • Proteins, peptides
  • Oligonucleotides
  • Oligosaccharides
  • Supramolecular compounds

Applications

  • MALDI-MS of intact proteins
  • Peptid mass fingerprints
  • MALDI-MSMS
  • MALDI-Imaging
  • TLC-MALDI-MS