Deutsch Intern
    Workgroup Seibel


    Fink J., Seibel J., Avota E. 

    Detection of Functionalized Sphingolipid Analogs in Detergent-Resistant Membranes of Immune Cells

    In: Bieberich E. (eds) Lipid Rafts. Methods in Molecular Biology, vol 2187. Humana, New York, NY




    M. Zimniak, L. Kirschner, H. Hilpert, J. Seibel, J. Bodem

    The serotonin reuptake inhibitor Fluoxetine inhibits SARS-CoV-2

    bioRxiv, doi: https://doi.org/10.1101/2020.06.14.150490 ,2020



    N. Wolf, L. Kersting, C. Herok, C. Mihm, J. Seibel

    High Yielding Water-Soluble Asymmetric Cyanine Dyes for Labeling Applications

    J. Org. Chem.. 2020, accepted for publication


    P.J. Smith, M.E. Ortiz-Soto, C. Roth, W. J. Barnes, J. Seibel, B. R. Urbanowicz, F. Pfrengle

    Enzymatic Synthesis of Artificial Polysaccharides

    ACS Sustainable Chem. Eng. 2020, accepted for publication


    K. Rajeeve, N. Vollmuth, S. Janaki-Raman, T. Wulff, M. Schmalhofer, W. Schmitz, A. Baluapuri, C. Huber, J. Fink, F. R. Dejure, E. Wolf, W. Eisenreich, A. Schulze, J. Seibel, T. Rudel

    Reprogramming of host glutamine metabolism during Chlamydia trachomatis infection and its key role in peptidoglycan synthesis

    Nature Microbiology 2020, accepted for publication


    ME Ortiz-Soto, JR Porras-Domínguez, ME Rodríguez-Alegría, LA Morales-Moreno, A Díaz-Vilchis, E Rudiño-Piñera, NE Beltrán-Hernandez, HM Rivera, J Seibel, A López Munguía

    Implications of the mutation S164A on Bacillus subtilis levansucrase product specificity and insights into protein interactions acting upon levan synthesis

    Int. J. Biol. Macromolecules 2020, 161, 898-908.




    F. Solger, T. C. Kunz, J. Fink, F. Schumacher, K. Paprotka, P. Pfister, F. Hagen, B. Kleuser, J. Seibel, T. Rudel

    A role of sphingosine in the intracellular survival of Neisseria gonorrhoeae

    Front. Cell. Infect. Microbiol. 2020, 10250, doi.10.3389/fcimb.2020.00215


    Judith Lang, Patrick Bohn, Hilal Bhat, Holger Jastrow, Bernd Walkenfort, Feyza Cansiz, Julian Fink, Michael Bauer, Dominik Olszewski, Ana Ramos-Nascimento, Vikas Duhan, Sarah-Kim Friedrich, Katrin Anne Becker, Adalbert Krawczyk, Michael J. Edwards, Andreas Burchert, Magdalena Huber, Justa Friebus-Kardash, Joachim R. Göthert, Cornelia Hardt, Hans Christian Probst, Fabian Schumacher, Karl Köhrer, Burkhard Kleuser, Eduard B. Babiychuk, Beate Sodeik, Jürgen Seibel, Urs F. Greber, Philipp A. Lang, Erich Gulbins & Karl S. Lang

    Acid ceramidase of macrophages traps herpes simplex virus in multivesicular bodies and protects from severe disease

    Nat Commun 2020, 11, 1338doi:10.1038/s41467-020-15072-8


    M. E. Ortiz-Soto, S. Reising, A. Schlosser, J. Seibel

    Structural and functional role of disulphide bonds and substrate binding residues of the human beta-galactoside alpha-2,3-sialyltransferase 1 (hST3Gal1)

    Sci Rep 2019, 9, 17993, doi:10.1038/s41598-019-54384-8


    C. Hollmann, T. Wiese, F. Dennstädt, J. Fink, J. Schneider-Schaulies, N. Beyersdorf

    Translational Approaches Targeting Ceramide Generation From Sphingomyelin in T Cells to Modulate Immunity in Humans

    Fron. Immunol. 2019https://doi.org/10.3389/fimmu.2019.02363.


    T Wiese, F Dennstaedt, C Hollmann, S Stonawski, C Wurst, J Fink, J Seibel, J Rohr, M Buttmann, A Menke, J Schneider-Schaulies, N Beyersdorf

    CD4+ Foxp3+ regulatory T cell-mediated immunomodulation by pharmacological inhibition of the acid sphingomyelinase in humans



    V. Merz,  J. Lenhart,  Y. Vonhausen,  M. E. Ortiz‐Soto,  J. Seibel,  A. Krueger

    Nanodiamond with Superior Protein Repulsion and Colloidal Stability in Physiological Media

    Small  2019, 15(48):e1901551. doi: 10.1002/smll.201901551.


    G. Beliu, A. Kurz, A. Kuhlemann, L. Behringer-Pliess, N. Wolf, J. Seibel, Zhen-Dan Shi, M. Schnermann, J. B. Grimm, L. D. Lavis, S. Doose, M. Sauer

    Bioorthogonal labeling with tetrazine-dyes for super-resolution microscopy

    Commun Biol. 2019, 2, 261. doi: 10.1038/s42003-019-0518-z. eCollection 2019.


    M. E. Ortiz-Soto, J.R. Porras-Domínguez, J. Seibel, A. López-munguía

    A close look at the structural features and reaction conditions that modulate the synthesis of low and high molecular weight fructans by levansucrases

    Carbohydrat Polymers 2019, 219, 130-142.


    C. Possiel, M. E. Ortiz-Soto, J. Ertl, A. Münch, A. Vogel, R. Schmiedel, J. Seibel

    Exploring the sequence variability of polymerization-involved residues in the production of levan- and inulin-type fructooligosaccharides with a levansucrase

    Scientific Reports 2019, 9, 7720.


    L. Irsheid, T. Wehler, C. Borek, W. Kiefer, R. Brenk, M. E. Ortiz-Soto, J. Seibel, T. Schirmeister

    Identification of a potential allosteric site of Golgi α-mannosidase II using computer-aided drug design

    PLoS ONE  2019, 14 (5), e0216132.


    J. Ertl, M. E. Ortiz-Soto, T. A. Le, J. Bechold, J. Shan, J. Teßmar, B. Engels, J. Seibel

    Tuning the product spectrum of a glycoside hydrolase enzyme by a combination of site‐directed mutagenesis and tyrosine‐specific chemical modification

    Chemistry - A European Journal 2019, 25, 6533-6541.



    M. Gutmann, J. Bechold, J. Seibel, L. Meinel, T. Lühmann

    Metabolic glycoengineering of cell derived matrices and cell surfaces – a combination of key principles and step-to-step procedures

    ACS Biomater. Sci. Eng.  2019, 5 (1), 215–233.



    D. Kaiser, J. M. Winne, M. E. Ortiz-Soto, J. Seibel, T. Anh Le, B. Engels

    Mechanistical insights into the bioconjugation
reaction of Triazolinediones with tyrosine

    J. Org. Chem. 2018, 83, 10248–10260.


    M. Kraus, C. Grimm, J. Seibel

    Reversibility of a Point Mutation Induced Domain Shift: Expanding the Conformational Space of a Sucrose Phosphorylase

    Scientific Reports  2018, 8, 10490.



    M. E. Ortiz-Soto, J. Ertl, J. Mut, J. Adelmann, T. A. Le, J. Shan, J. Teßmar, A. Schlosser, B. Engels, J. Seibel

    Product-oriented chemical surface modification of a levansucrase (SacB) via an ene-type reaction 

    Chem. Sci.  2018, 9, 5312-5321.




    J. Fink, J. Seibel

    Click reactions with functional Sphingolipids

    Biol. Chem. 2018399(10), 1157-1168.



    M. Gutmann, A. Braun, J. Seibel, T. Lühmann

    Bioorthogonal modification of cell derived matrices by metabolic glyco-engineering

    ACS Biomaterials Science & Engineering 2018, 4, 1300-1306.


    J. Seibel, C. Grimm, J. Bechold

    Mit Zucker gegen Bösartiges

    Nachrichten aus der Chemie 2018, 66 (1), 30-31.


    J. Becam, T. Walter, A. Burgert, J. Schlegel, M. Sauer, J. Seibel, A. Schubert-Unkmeir

    Antibacterial activity of ceramide and ceramide analogs against pathogenic Neisseria

    Scientific Reports 2017, 7, Article number: 17627 (2017)





    M. Kraus, C. Grimm, J. Seibel

    Switching enzyme specificity from phosphate to resveratrol glucosylation

    Chem. Commun. 2017, 53, 12181 - 12184.


    C. Possiel, A. Bäuerle, J. Seibel

    A novel Chemoenzymatic route to a new class of Sucrose esters

    Eur. J. Org. Chem. 2017, 6335–6337.

    ­­J. Görl, C. Possiel, C. Sotriffer, J. Seibel,

    Extending the Scope of GTFR Glucosylation Reactions with Tosylated Substrates for Rare Sugars Synthesis

    ChemBioChem 201718, 2012-2015.



    M. Elena Ortiz-Soto, C. Possiel, J. Görl, A. Vogel, R. Schmiedel, J. Seibel

    Impaired coordination of nucleophile and increased hydrophobicity in the +1 subsite shift levansucrase activity towards transfructosylation

    Glycobiology 201727, 755-765.



    N. Bertleff-Zieschang, J. Bechold, C. Grimm, M. Reutlinger, P. Schneider, G. Schneider, J. Seibel

    Exploring the structural space of galectin-1-ligand interaction

    ChemBioChem 2017, 18, 1477-1481.


    T. Walter,  J. Schlegel,  A. Burgert, A. Kurz,  J. Seibel and M. Sauer

    Incorporation studies of clickable ceramides in Jurkat cell plasma membranes

    Chem. Commun2017, 53, 6836-6839.


    M. Ortiz-Soto, J. Seibel

    Molecular Engineering of Enzymes
    Applied Bioengineering: Innovations and Future Directions 2017

    Stephan Wawra, Philipp Fesel, Heidi Widmer, Malte Timm, Jürgen Seibel, Lisa Leson, Leona Kesseler, Robin Nostadt, Magdalena Hilbert, Gregor Langen & Alga Zuccaro
    The fungal-specific β-glucan-binding lectin FGB1 alters cell-wall composition and suppresses glucan-triggered immunity in plants
    Nat. Commun. 2016, 7, 13188.


    A. Mertsch, S. Letschert, E. Memmel, M. Sauer, J. Seibel
    Synthesis and application of water soluble, photoswitchable cyanine dyes for bioorthogonal labeling of cell-surface carbohydrates
    Biosciences – Zeitschrift für Naturforschung C 2016, 71, 347–354.


    J. Seibel
    ZNC opens a new chapter focussing on the emerging field of natural and natural-like compounds
    Biosciences – Zeitschrift für Naturforschung C 2016, 71, 93


    M. Ortiz-Soto, J. Seibel
    From gene to product: tailor-made oligosaccharides and polysaccharides by enzyme and substrate engineering
    Carbohydrate-Modifying Biocatalysts 2016, 535


    T. Walter, L. Collenburg, L. Japtok, B. Kleuser, S. Schneider-Schaulies, N. Müller, J. Becam, A. Schubert-Unkmeir, J. N. Konge, E. Bieberich and J. Seibel
    Incorporation and visualization of azido-functionalized N-oleoyl serinol in Jurkat cells, mouse brain astrocytes, 3T3 fibroblasts and human brain microvascular endothelial cells
    Chem. Commun. 2016, 52, 8612-8614


    M. Ortiz-Soto, J. Seibel
    Expression of Functional Human Sialyltransferases ST3Gal1 and ST6Gal1 in Escherichia coli
    PLoS One 2016,  11, e0155410.


    L. Collenburg, T. Walter, A. Burgert, N. Müller, J. Seibel, L. Japtok, B. Kleuser, M. Sauer, S. Schneider-Schaulies
    A Functionalized Sphingolipid Analogue for Studying Redistribution during Activation in Living T Cells
    J. Immunol. 2016, 196, 3951-3962.


    M. Kraus, J. Görl, M. Timm, J. Seibel
    Synthesis of the rare disaccharide nigerose by structure-based design of a phosphorylase mutant with altered regioselectivity
    Chem. Commun. 2016, 52, 4625-4627.


    M. Gutmann, E. Memmel, A. Braun, J. Seibel, L. Meinel, T. Lühmann
    Biocompatible azide alkyne"click" reactions for surface decoration of glyco-engineered cells

    ChemBioChem 2016, 17, 866-875. 

    M. Kraus, C. Grimm, J. Seibel
    Redesign of the active site of sucrose phosphorylase by a clash induced cascade of loop shifts

    ChemBioChem 2016, 17, 33–36.

    C. Fleck, E. Memmel, M. Fölsing, B. Poll, T. Hackl, J. Seibel, W. Maison
    Synthesis and evaluation of neoglycoconjugates based on adamantyl scaffolds.
    Eur. J. Org. Chem. 2015, 8, 1696–1710.

    E. Memmel, J. Seibel
    Metabolic Engineering of Bacteria.
    in Proceedings of the 3rd Beilstein Glyco-Bioinformatics Symposium 2013 (Herausgeber M. G. Hicks, C. Kettner, P. Seeberger)
    Beilstein Institut, Frankfurt/Main 2014, 1-9.

    D. Claes, E. Memmel, M. Holzapfel, J. Seibel, W. Maison
    High-Affinity Carbohydrate Binding by Trimeric Benzoboroxoles Measured on Carbohydrate Arrays.

    ChemBioChem 2014, 15, 2450–2457.

    S. Letschert, A. Göhler, C. Franke, N. Bertleff-Zieschang, E. Memmel, S. Doose, J. Seibel, M. Sauer
    Super-Resolution Imaging of Plasma Membrane Glycans.
    Angew. Chem. Int. Ed. 2014, 53(41), 10921–10924.

    U. T. Bornscheuer, K. Buchholz, J. Seibel
    Enzymatic degradation of (ligno-)celluloses.
    Angew. Chem. Int. Ed. 2014, 53(41), 10876–10893.

    S. Bank, E. Heller, E. Memmel, J. Seibel, U. Holzgrabe, P. Kapková
    Matrix-assisted laser desorption/ionization tandem mass spectrometry of N-glycans derivatized with isonicotinic hydrazide and its biotinylated form.
    Rapid Commun. Mass Spectrom. 2014, 28(15), 1745–1756.


    M. E. Ortiz-Soto, J. Seibel
    Biotechnological Synthesis and Transformation of Valuable Sugars in the Food and Pharmaceutical Industry.
    Current Organic Chemistry 2014, 18, 964–986.